Showing: 1801 - 1820 of 1875 results
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Plasmid#156556PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF9 (TNFRSF9 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ICAM2-Fc(DAPA)-AviTag-6xHis
Plasmid#156610PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertICAM2 (ICAM2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
dCas9-p300 HAT
Plasmid#179544Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-p300 PHD-HAT
Plasmid#179545Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-p300 RING-PHD-HAT
Plasmid#179546Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP HAT
Plasmid#179547Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP PHD-HAT
Plasmid#179548Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP RING-PHD-HAT
Plasmid#179549Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
dCas9-GCN5 core
Plasmid#179551Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187960PurposeAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187963PurposemAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertsAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linker and T2AExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTR-373 tNGFR-P2A-IL7R
Plasmid#186058PurposeKnockin of truncated NGFR to target geneDepositorInsertIL7R (IL7R Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailabilityAcademic Institutions and Nonprofits only -
pIG-732pUC19-tNGFR-P2A-STIM1
Plasmid#186119PurposeKnockin of truncated NGFR to target geneDepositorInsertSTIM1 (STIM1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-INPP4B-mCherry
Plasmid#184055PurposeLysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.DepositorUseTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLY162
Plasmid#184138PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R1, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only -
pLY164
Plasmid#184140PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R3, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only -
pLY163
Plasmid#184141PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R2, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only -
pLY168
Plasmid#184142PurposeReporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-Ar2, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only -
pLY300
Plasmid#184158PurposeReporter circuit for hijacking small RNA oxyS as CRISPR RNADepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-oxyS-86, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only -
pLY305
Plasmid#184159PurposeReporter circuit for hijacking mRNA of zraP as CRISPR RNADepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
mutsfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationK140N, Mutations D10A and H840A on WT cas9. Synon…PromoterPcon, PpspA-zraP-22, PrhaB, and PtetAvailabilityAcademic Institutions and Nonprofits only
Showing: 1801 - 1820 of 1875 results