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pAGM1482

Plasmid

#50295

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45195

Plasmid

#50275

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH85281

Plasmid

#50272

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) no-tags pET

Plasmid

#8493

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Use

Tags

Expression

Bacterial

Mutation

makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pAGM5343

Plasmid

#50297

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I Y248&249F

Plasmid

#22189

Depositor

Ananda Roy

Article

Novina et al J Biol Chem. 1998 Dec 11. 273(50):33443-

Insert

TFII-I (GTF2I Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines.

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Use

Tags

HisTag

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Use

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-NLS-mCherry

Plasmid

#231890

Purpose

mCherry-tagged nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

mCherry-SV40 NLS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226291

Purpose

Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR

Use

Lentiviral

Tags

2xMyc-tdMCP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Use

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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RNA-Protein Landing Pad

Plasmid

#226162

Purpose

Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

mTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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2COX8-BmTyr

Plasmid

#222943

Purpose

mitochondrion-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

2COX8-BmTyr-Flag

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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