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Plasmid#155151PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationadded Streptavidin tag on MlaF N-terminusPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2074
Plasmid#155155PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaB_T52EPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2076
Plasmid#155156PurposeExpresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_K47APromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2077
Plasmid#155157PurposeExpresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_E170QPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2078
Plasmid#155158PurposeExpresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_Y261APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2079
Plasmid#155159PurposeExpresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_L265APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yes-10kb
Plasmid#112996PurposeP element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genomeDepositorInsert10Kb DAFC-62D amplicon
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSABAD518
Plasmid#46374DepositorInsertTvoVMA intein, inactive
UseTagsH6-GB1 and SH3ExpressionBacterialMutationN186A, T+1A mutations, inactivePromoterpBADAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG-deltaA
Plasmid#33151DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
UseTagsLacZExpressionYeastMutationThe RP51A sequence was modified by digestion with…PromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-368.wt
Plasmid#13531DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206 and 369-479PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
KCC2G_HUMAN_D0
Plasmid#79711PurposeThis plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2G (CAMK2G Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertABL1 (ABL1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R
Plasmid#163990PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationE1461RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB1 (EPHB1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only