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Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD3
Plasmid#226405PurposeExpression of human MOSPD3 fused to EGFP in mammalian cellsDepositorInsertMOSPD3 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmax
Plasmid#206883PurposeExpresses FLAG-tagged PEmax fused to Gag through a linker sequenceDepositorInsertPEmax
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-mScarlet-WPRE
Plasmid#99280PurposeCan be used to generate AAV virus that will express mScarlet in the presence of CreDepositorInsertmScarlet
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-Flag-DHHC5
Plasmid#85812PurposeTo rescue the expression of DHHC5 in the knockdown cell linesDepositorInsertZDHHC5 (Zdhhc5 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOSV805
Plasmid#126598Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn-FAS-DO-ReaChR-mCitrine-WPRE
Plasmid#64035PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructsDepositorInserthSyn-FAS-DO-ReaChR-mCitrine-WPRE
UseAAVTagsReaChR tagged with mCitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100853PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Syntaxin1A
Plasmid#170871PurposeMammalian expression of His-GFP-Syntaxin1A.DepositorInsertSyntaxin 1A (Stx1a Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only