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Plasmid#195238PurposeMammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-GPI
Plasmid#65296Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFTu-His
Plasmid#124127PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor Tu (tufB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAC569
Plasmid#195241PurposeMammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFG-His
Plasmid#124126PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor G (fusA Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-mNeonGreen-GW
Plasmid#209352PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF2
Plasmid#124124PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 2 (infB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorInsertSeryl-tRNA ligase (serS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAsparaginyl-tRNA ligase (asnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF1
Plasmid#124123PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 1 (infA Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MTF-His
Plasmid#124133PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionyl-tRNA formyltransferase (fmt Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-His-ArgRS
Plasmid#124104PurposeBacterial expression plasmid for PURE system componentsDepositorInsertArgin+I4 ligase (argS Escherichia coli)
UseTags10x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-RF3
Plasmid#124131PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRelease factor 3 (prfC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF3
Plasmid#124125PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 3 (infC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-ThrRS
Plasmid#124119PurposeBacterial expression plasmid for PURE system componentsDepositorInsertThreonyl-tRNA ligase (thrS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFTs-His
Plasmid#124128PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor Ts (tsf Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-NDK
Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorInsertNucleotide diphosphate kinase (ndk Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only