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Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ABP1-iGFPx6
Plasmid#177099PurposeExpresses Abp1-iGFPx6 in yeast cellsDepositorInsertABP1 (ABP1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ENT5-HaloTag
Plasmid#177102PurposeExpresses Ent5-HaloTag in yeast cellsDepositorInsertENT5 (ENT5 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP2-STE13
Plasmid#177106PurposeExpresses msGFP2-Ste13 in yeast cellsDepositorInsertSTE13 (STE13 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorInsertFRBx2 (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-STV1-iGFP
Plasmid#177111PurposeExpresses Stv1-iGFP in yeast cellsDepositorInsertSTV1 (STV1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXY1-spc-ptb
Plasmid#90489PurposepXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS16-iGFPx6
Plasmid#105275PurposePlasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.DepositorInsertVps16 portion (VPS16 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
UseTagsHaloTagExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
UseTagsExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pI12
Plasmid#24273DepositorInsertmodified adenovirus exons and introns
UseT7 for in vitro transcriptionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1a
Plasmid#164956PurposeZebrafish kcnn1a gene CDSDepositorInsertkcnn1a (kcnn1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSec7-iGFPx6
Plasmid#115424PurposeExpress Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-iGFPx6
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF236
Plasmid#46377DepositorInsertNpuDnaE intein delta variant, inactive
UseTagsH6-Smt3ExpressionBacterialMutationC1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…PromoterT7Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only