Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1781 - 1800 of 1937 results

  2. pNHT7

    Plasmid
    #186753
    Purpose
    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Type
    Empty backbone
    Use
    Synthetic Biology; Cloning backbone
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    186753-plasmid-map-sequence-id-375095

    Enlarge

  10. p-OiRS3GG

    Plasmid
    #98589
    Purpose
    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
    Depositor
    Lydia Contreras
    Article
    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552
    Inserts
    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD and pLtetO
    Availability
    Academic Institutions and Nonprofits only
    98589-plasmid-map-sequence-id-194130

    Enlarge

  11. pEF004-sgRNA-shuffle-in

    Plasmid
    #104440
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    U6 promoter
    Availability
    Academic Institutions and Nonprofits only
    104440-plasmid-map-sequence-id-201143

    Enlarge

  12. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid
    #123180
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123180-plasmid-map-sequence-id-241283

    Enlarge

  13. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123181-plasmid-map-sequence-id-241286

    Enlarge

  14. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid
    #123182
    Purpose
    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123182-plasmid-map-sequence-id-241413

    Enlarge

  15. pSB61 - pL0_V2 (CDS1)

    Plasmid
    #123183
    Purpose
    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123183-plasmid-map-sequence-id-241416

    Enlarge

  16. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

    Enlarge

  17. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-329408

    Enlarge

  18. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA Ser promoter
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

    Enlarge

Showing: 1781 - 1800 of 1937 results