-
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
mMaroon-RTK-mMaroon
Plasmid#84359PurposeFRET acceptor for RhoA donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-Rtk--mMaroon
UseTagsmMaroon flanking Rtk domain on N and C terminusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ONECUT2
Plasmid#135738PurposeDonor vector for 3' FLAG tag of human ONECUT2DepositorInsertONECUT2 Homology arms (ONECUT2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Actb-mRuby3 KI
Plasmid#183437PurposeFlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and mRuby3 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC5 Tubb3-GFP KI
Plasmid#183440PurposeFlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)DepositorInsertgRNA and GFP donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TwinStrep-GFP11
Plasmid#153514PurposeTwinStrep-GFP11 C-terminal Tag Donor PlasmidDepositorInsertTwinStrep-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD-mW-attPCC-FRT (pTL717)
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5741
Plasmid#128722PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcV-hopV1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-4xGFP KI
Plasmid#183445PurposeGFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and 4xGFP donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L4-pTF-L1R
Plasmid#45954DepositorInsertpTF promoter
UseGateway donor vectorTagsExpressionMutationPromoterTET optimizedAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF503
Plasmid#135735PurposeDonor vector for 3' FLAG tag of human ZNF503DepositorInsertZNF503 Homology arms (ZNF503 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_OctaHis-GFP11
Plasmid#153509PurposeOctaHis-GFP11 C-terminal Tag Donor PlasmidDepositorInsertOctaHis-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF331
Plasmid#135726PurposeDonor vector for 3' FLAG tag of human ZNF331DepositorInsertZNF331 Homology arms (ZNF331 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -