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Plasmid#47077PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site, GFP, and HisExpressionMutationPromoterNIS (nisin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only
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PresentER-Cassette mCherry
Plasmid#102943PurposePresentER with removable cassette for cloning of MHC-I antigen (mCherry version)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF003
Plasmid#48844PurposeProvides a D-alanine resistance cassette as GreenGate module.DepositorInsertpMAS::D-AlanineR::tMAS
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL2-(GGS)4GSG
Plasmid#137099PurposeiFLinkC Linker cloningDepositorInsert(GGS)4GSG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-G(P)10G
Plasmid#137105PurposeiFLinkC Linker cloningDepositorInsertG(P)10G
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-G(P)7G
Plasmid#137100PurposeiFLinkC Linker cloningDepositorInsertG(P)7G
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-(GGS)2G
Plasmid#137097PurposeiFLinkC Linker cloningDepositorInsert(GGS)2G
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-GGASPAGG
Plasmid#137102PurposeiFLinkC Linker cloningDepositorInsertGGASPAGG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-GGASPAAPAPAG
Plasmid#137103PurposeiFLinkC Linker cloningDepositorInsertGGASPAAPAPAG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2- GL(GGGS)3ETG
Plasmid#137106PurposeiFLinkC Linker cloningDepositorInsertGL(GGGS)3ETG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-G(TP)4TG
Plasmid#137101PurposeiFLinkC Linker cloningDepositorInsertG(TP)4TG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-(GGS)3G
Plasmid#137098PurposeiFLinkC Linker cloningDepositorInsert(GGS)4G
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-GSPAG
Plasmid#137096PurposeiFLinkC Linker cloningDepositorInsertGSPAG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGGB003
Plasmid#48821PurposeProvides a default dummy as GreenGate N-terminal tag module.DepositorInsertB-Dummy
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXCA3H
Plasmid#47072PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGF005
Plasmid#48846PurposeProvides a hygromycin resistance cassette as GreenGate module.DepositorInsertpUBQ10::HygromycinR::tOCS
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGD002
Plasmid#48834PurposeProvides a default dummy as GreenGate C-terminal tag module.DepositorInsertD-Dummy
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only