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Plasmid#176793PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert405
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A
Plasmid#128944Purposeexpresses R480A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAG324V
Plasmid#128947Purposeexpresses G324V mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
10xHis-ataxin-3 *SIM-HA
Plasmid#89983PurposeExpresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
UseTags10xHis and HAExpressionMammalianMutationmutated aa 162IFVV to 162AFAAPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *SIM
Plasmid#89979PurposeExpresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationmutated 162IFVV to 162AFAAPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI
Plasmid#131479PurposeEndogenous tagging of βactin: N-terminal (amino acid position: D2)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa-FRB-3myc-KIF1B beta tail 387-1770
Plasmid#64287PurposeKIF1B beta aa 387-1770 with N-terminal FRB and triple myc tagsDepositorInsertKIF1B beta (Kif1b Mouse, Rat)
UseTags3myc and FRBExpressionMammalianMutationaa 387-1770, small portion of 5'end matches …Promoterchicken Beta ActinAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mIFP12-FilaminA-C-14
Plasmid#56253PurposeLocalization: Actin/Focal Adhesions, Excitation: 683, Emission: 704DepositorInsertFilaminA (FLNA Human)
UseTagsmIFP12ExpressionMammalianMutationD2626HPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
1529_pAAV-CB-FKBP12-BirA-HA
Plasmid#208200PurposeExpress the murine FKBP12-BirA-HA fusion protein in AAV vectorDepositorInsertFkbp12 (Fkbp1a Mouse)
UseAAVTagsExpressionMutationPromoterChicken beta-actinAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-HA
Plasmid#27553DepositorTypeEmpty backboneUseTags3x HAExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
F3/pRC-CMV
Plasmid#197326PurposePlasmid expressing an antigen targeting the Contactin/F3 scaffold proteinDepositorInsertContactin/F3 (Cntn1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only