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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-hCMV1-hNANOG-IV

Plasmid

#60432

Purpose

tet-inducible human NANOG

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

NANOG (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-VSV-G-HiBiT

Plasmid

#162594

Purpose

C-terminally HiBiT-tagged VSV-G under CMV promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

VSV-G

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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NaChBac pTracer CMV2

Plasmid

#60835

Purpose

Mammalian expression plasmid containing NaChBac gene with an EGFP reporter

Depositor

David Clapham

Article

Ren et al Science. 2001 Dec 14;294(5550):2372-5.

Insert

NaChBac

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-DPC4

Plasmid

#14039

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Use

Tags

Expression

Mutation

Promoter

tight TRE

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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4xHRE_minSV40-sfGFP-CMV_dsRed

Plasmid

#117400

Purpose

sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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PCLP-2/pCMV (100)

Plasmid

#24421

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pR26-CMVconst-mBMP7

Plasmid

#127375

Purpose

Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV enhancer/promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-eBE-YE

Plasmid

#107689

Purpose

Expresses dLbCpf1-eBE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE-YE

Use

Tags

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-GFP-W

Plasmid

#196046

Purpose

lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad1

Plasmid

#11735

Depositor

Jeff Wrana

Article

Hoodless et al Cell. 1996 May 17. 85(4):489-500.

Insert

SMAD1 (SMAD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
MXS_CMV::ZeoR-bGHpA

Plasmid

#62441

Purpose

MXS_chaining vector with CMV::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-CARP2

Plasmid

#16013

Depositor

Wafik El-Deiry

Article

McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

Insert

CARP2 (RFFL Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pX551-miniCMV-SaCas9

Plasmid

#107032

Purpose

AAV plasmid expressing SaCas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

SaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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