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Plasmid#11483DepositorInsertMmUbc6 (Ube2j2 Mouse)
UseTagsHAExpressionMammalianMutationcys 94 mutated to serPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
gRNA_tdtomato_reporter_1_ST1_2x_binding
Plasmid#79368PurposeAssays activity of transcriptional activators fused to ST1 Cas9DepositorInserttdtomato
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-Pacsin2
Plasmid#109587PurposeMultisite gateway vector for 3' tagging with Pacsin2DepositorInsertPacsin2 (pacsin2 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-Scaffold
Plasmid#78906PurposeVector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
UseTags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 short UTR
Plasmid#21644DepositorInsertCyclin D2 short UTR (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSCV Cyclin D2-long-mut
Plasmid#21657DepositorInsertCyclin D2 (CCND2 Human)
UseRetroviralTagsExpressionMammalianMutationmutated miR-15/16 and let-7 binding sitesPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
3JZN
Plasmid#60123PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEED:APC056-C06:C32050 (EED EED:APC056-C06:C32050, Human)
UseTags6xHis and GSTExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCCr4
Plasmid#79252PurposeExpresses EGFP-tagged DmCCr4 in S2 cellsDepositorInsertDmCaf40 (twin Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GRM1
Plasmid#25353PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM1-C254S:L28-G518 (GRM1 Human)
UseTagsHisExpressionInsectMutationC254S. Contains amino acids L28-G518.PromoterAvailable sinceJuly 29, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS cFgfr2 (CT#221)
Plasmid#14003DepositorInsertFgfr2 (FGFR2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hoxd10 probe
Plasmid#34712DepositorInsertHoxd10 (Hoxd10 Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 LxCxE HA
Plasmid#8957DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationDeletion of LxCxE motifPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_3_TOPO_TA-ms2-VP64
Plasmid#79371PurposeRecruits VP64 to a compatible gRNA for transcriptional activationDepositorInsertMS2-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:MCP-VP64
Plasmid#78904PurposeExpresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertMS2:VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceMarch 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 K112E HA
Plasmid#8953DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationK112E: point mutation in cyclin boxPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only