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Plasmid#228689PurposeN terminal dual protein A affinity tag with TEV cleavage siteDepositorInsert2xProtein A-TEV
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.010
Plasmid#228696PurposeC terminal Strep affinity tag with TEV cleavage siteDepositorInsertTEV-Strep
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.009
Plasmid#228695PurposeN terminal Strep affinity tag with TEV cleavage siteDepositorInsertStrep-TEV
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK635
Plasmid#72473PurposepET28a modified to contain TEV protease fusion tag instead of T7 tagDepositorTypeEmpty backboneUseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AF20-double-tag
Plasmid#108389PurposeExpress human PC1DepositorInsertPKD1 (PKD1 Human)
UseTagseGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CC-B
Plasmid#196479PurposeProtein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCCDepositorInsertB-CC-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GST
Plasmid#62329PurposeE. coli pACYC based expression vector with N-terminal TEV cleavable GST-tagDepositorTypeEmpty backboneUseTagsGlutathione S-transferase (GST) tag and TEV prote…ExpressionBacterialMutationPromoterT7-lac0Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-iLOVf
Plasmid#63722PurposeLIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
UseTagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
thoc5.018.389
Plasmid#137266PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertthoc5 (THOC5 Human)
UseTags(His)6-TEVExpressionBacterialMutation018-389PromoterAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
NKAP.001.140
Plasmid#137206PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertNKAP (NKAP Human)
UseTags(His)6-TEVExpressionBacterialMutation001-140PromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBS-864
Plasmid#218018PurposeGST-tag fused to TEV Protease cleavage site for protein purification and cleavageDepositorInsertGST-tag
UseSynthetic BiologyTagsTEV protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and td…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mR…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB-CT10HF-LIC
Plasmid#39191PurposeSGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirusTagsTEV-His-FLAGExpressionMutationPromoterpolyhedrinAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (H6-mClover)
Plasmid#86931PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mCloverDepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1Q1
Plasmid#97326PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1Q1
UseTags6xHis - TEVExpressionBacterialMutationAddition of Q residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.169.S34E
Plasmid#137643PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTags(His)6-TEVExpressionBacterialMutation001-169-S34EPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only