Addgene

Showing: 161 - 180 of 4470 results

Results per page:

Sort results by:

pCri-17a

Plasmid

#61325

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-ProteoTuner DD

Plasmid

#67181

Purpose

CRISPaint universal donor for fusion tagging allowing inducible destabilization

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

ProteoTuner DD

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-HA-PuroR

Plasmid

#80959

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA tag

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPomyces-FnCpf1

Plasmid

#129554

Purpose

Contains codon-optimized FnCpf1 and gRNA for streptomyces

Depositor

Mingzi M. Zhang, Fong Tian Wong

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

FnCpf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Tags

Expression

Mutation

Promoter

Available since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPomyces-SaCas9

Plasmid

#129553

Purpose

Contains codon-optimized SaCas9 and gRNA for streptomyces

Depositor

Mingzi M. Zhang, Fong Tian Wong

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

SaCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCralbp-DsRed

Plasmid

#11158

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Cralbp promoter (Rlbp1 Mouse)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
shha-pCRII

Plasmid

#68962

Purpose

for antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

Insert

shha (shha Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pcrDNA3.1-PRKACA

Plasmid

#100890

Purpose

Mammalian expression of PRKACA

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)

Use

Tags

Expression

Mammalian

Mutation

Isoform 1 wild type

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR3.1-OPNa

Plasmid

#107980

Purpose

expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

full-length osteopontin (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCruzHA SIRT1

Plasmid

#10962

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-TagRFP

Plasmid

#67167

Purpose

CRISPaint universal donor for TagRFP fusion tagging

Depositor

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagRFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

Plasmid

#127177

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Expression

Mutation

Promoter

None

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Article

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-TagBFP

Plasmid

#67168

Purpose

CRISPaint universal donor for TagBFP fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagBFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR eGFPCreERT2 L1-R5

Plasmid

#43961

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

eGFP-CreERT2

Use

Multisite gateway entr clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge