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pRSET-A2-77p-Venus

Plasmid

#64856

Purpose

Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

Depositor

Sriram Neelamegham

Article

Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

Insert

VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)

Use

Tags

7X His and Venus

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-25u

Plasmid

#14480

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W230A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Use

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pRSET-GST-05230

Plasmid

#67874

Purpose

E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-100u

Plasmid

#13539

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII12Pglu-600uDelta4

Plasmid

#13567

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 4aa linker

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Promoter

Available since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1m

Plasmid

#13551

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207W (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207W, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-225u

Plasmid

#14481

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W62A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pRSETb-LSS-mEGFP

Plasmid

#112942

Purpose

Bacterial expression vector for LSS-EGFP

Depositor

Dorus Gadella

Insert

LSSS-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-LSS-SGFP2

Plasmid

#112939

Purpose

Bacterial expression vector for LSS-SGFP2

Depositor

Dorus Gadella

Insert

LSS-SGFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-control

Plasmid

#14482

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W340A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.b - pRSET

Plasmid

#193948

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.c - pRSET

Plasmid

#193949

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET QUEEN7mu R103A

Plasmid

#129372

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN7mu R103A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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