Addgene

We narrowed to 992 results for: nps

Showing: 161 - 180 of 992 results

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pCBEclos-cbei2385-g2-opt

Plasmid

#118215

Purpose

CRISPR-mediated base editing in Clostridium

Depositor

Sheng Yang

Article

Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

Insert

Apobec1, Casp nickase, UG1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptm

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

Plasmid

#190794

Purpose

Template vector to amplify pieces for creating multiplex sgRNA-arrays.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA-scaffold only

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23117

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01

Plasmid

#80046

Purpose

expression of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11 (1wa3)

Plasmid

#80122

Purpose

Palm1-Late1-I3-01-myc (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Use

Tags

Expression

Insect

Mutation

Promoter

bTub

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔM)

Plasmid

#80047

Purpose

Expression or G2A mutants of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18 (1wa3)

Plasmid

#80123

Purpose

PH-4GS-I3-01-myc-Late1 (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-25 (1wa3)

Plasmid

#80124

Purpose

I3-01p-myc-Late1-PH (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1+L2)

Plasmid

#80044

Purpose

Expression of EPN-01* mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01-(ΔLF)

Plasmid

#80052

Purpose

Expression of Vpr binding mutant EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaLF)

Use

Tags

Myc

Expression

Mammalian

Mutation

LF45AA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01(∆L1+∆L2)-His

Plasmid

#84511

Purpose

E.coli version of EPN01(∆L1+∆L2)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆L1+∆L2)-His

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_F

Plasmid

#237349

Purpose

Expression plasmid for RSV Long F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV Long F from Genbank Accesion AY911262.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_F

Plasmid

#237351

Purpose

Expression plasmid for RSV A2 F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV A2 F human codon optimized from Genbank Accesion QGW56794.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B1_F

Plasmid

#237353

Purpose

Expression plasmid for RSV B1 F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B1 F human codon optimized from Genbank Accesion AF013254

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Ydf NanoLuc

Plasmid

#225656

Purpose

Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NanoLuciferase

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Mutation

Promoter

PydfJ115

Available since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6.3

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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