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Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4
Plasmid#204692PurposeThis plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.DepositorInsertBRD4 (BRD4 Human)
UseTagsmNeonGreenExpressionMammalianMutationn/aPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen-WPRE
Plasmid#193341PurposeAAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-URA3
Plasmid#168059PurposeReplaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Etanercept
Plasmid#182421PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusionDepositorInsertVNp-mNeongreen-Etanercept (TNFRSF1B Human)
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT-mRuby3
Plasmid#179225Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mNeongreen-Puro
Plasmid#169228PurposeTargeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen-P2A-Puro
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7
Plasmid#182415PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusionDepositorInsertVNp-mNeongreen-DARPinOFF7
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen
Plasmid#205022Purposemini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mNeongreen-Blast
Plasmid#169229PurposeTargeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen-P2A-Blast
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNeonGreen-LEHD-NanoLuc
Plasmid#98289Purposecaspase-9 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆Nterm-mNeonGreen-IRESpuro2
Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mTurquoise2
Plasmid#125701PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2DepositorInsertymNeongreen-T2A-mTurquoise
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mNeongreen-T2A-mCherry
Plasmid#125698PurposeProduces equimolar expression of mNeongreen and mCherryDepositorInsertmNeongreen-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only