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Plasmid#166899Purposemammalian expression of Sar1b tagged with mOrange2DepositorInsertmOrange2
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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mApple-GPI-RUSH
Plasmid#166904PurposeRUSH cargo: Str-KDEL_IRES_SBP-mApple-GPIDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VPR
Plasmid#183918PurposeVPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VPR
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLV 4xSTAR-mTFP-NLS-blast
Plasmid#136257PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmTFP-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL R167W-pRc/CMV
Plasmid#22082DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant converting aa 167 from Arginine to T…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI-NLS-VP16
Plasmid#103837PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInserttagBFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag
Plasmid#29497DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI. PGK-puro
Plasmid#136266PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletIExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHMcc(delta16 PHM 356s)
Plasmid#25455DepositorInsertPHM (Pam Rat)
UseTagsExpressionMammalianMutationContains the catalytic core of PHM, including aa …PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-VHL(1-155)
Plasmid#29500DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsExpressionInsectMutationVHL(1-155)onlyPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec23A
Plasmid#166894Purposemammalian expression of Sec23A tagged with mCherryDepositorInsertSEC23A (SEC23A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-H79G-YFP
Plasmid#166898Purposemammalian expression of Sar1b-H79G tagged with YFPDepositorInsertSar1b-H79G
UseTagsYFPExpressionMammalianMutationH79 to GPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only