-
Plasmid#148514PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83DepositorInsertDmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJo55
Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-Hs4E-BP1
Plasmid#79438PurposeExpresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cellsDepositorInsertHs4E-BP1 (EIF4EBP1 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-Y54AM59A-GB1_AA
Plasmid#148284PurposeBacterial Expression of DmThor_50-83-Y54AM59ADepositorInsertDmThor_50-83-Y54AM59A (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor_Z
Plasmid#148188PurposeBacterial Expression of DmThorDepositorInsertDmThor (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-CLL78AAA_Z
Plasmid#148189PurposeBacterial Expression of DmThor-CLL78AAADepositorInsertDmThor-CLL78AAA (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84_Z
Plasmid#148190PurposeBacterial Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-GB1_Z
Plasmid#148192PurposeBacterial Expression of DmThorDepositorInsertDmThor (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-Y54AM59A_Z
Plasmid#148193PurposeBacterial Expression of DmThor-Y54AM59ADepositorInsertDmThor-Y54AM59A (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-YMCLLtoAAAAA_Z
Plasmid#148194PurposeBacterial Expression of DmThor-YMCLLtoAAAAADepositorInsertDmThor-YMCLLtoAAAAA (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z
Plasmid#148165PurposeInsect Expression of DmThor-CLL78AAADepositorInsertDmThor-CLL78AAA (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z
Plasmid#148166PurposeInsect Expression of DmThor-RGTtoAAADepositorInsertDmThor-RGTtoAAA (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z
Plasmid#148167PurposeInsect Expression of DmThor-Y54AM59ADepositorInsertDmThor-Y54AM59A (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-GB1_Z
Plasmid#148178PurposeBacterial Expression of DmThor_50-63DepositorInsertDmThor_50-63 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83_Z
Plasmid#148179PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_63-83-GB1_Z
Plasmid#148181PurposeBacterial Expression of DmThor_63-83DepositorInsertDmThor_63-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-DmThor_Z
Plasmid#148184PurposeBacterial Expression of DmThorDepositorInsertDmThor (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-YMCLLtoAAAAA_Z
Plasmid#148153PurposeInsect Expression of DmThor-YMCLLtoAAAAADepositorInsertDmThor-YMCLLtoAAAAA (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only