Addgene

We narrowed to 242 results for: clathrin

Showing: 161 - 180 of 242 results

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SH4-FRB-mRFP

Plasmid

#100741

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

Fyn

Use

Tags

FRB and mRFP

Expression

Mammalian

Mutation

SH4 domain only

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Syntaxin-4 d29

Plasmid

#44190

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 29 amino acids

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
YIplac211-iGFP-DRS2

Plasmid

#177104

Purpose

Expresses iGFP-Drs2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Syntaxin-4 d44

Plasmid

#44191

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 44 amino acids

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 d24

Plasmid

#44189

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 24 amino acids

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 d10

Plasmid

#44188

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 10 amino acids

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M1 (Y115A)

Plasmid

#44029

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Use

Tags

FRB-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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wt dynamin 1 pEGFP

Plasmid

#34680

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only
K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-Hs beta1

Plasmid

#45927

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Hs Beta1 adaptin (AP1B1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-shTACC3

Plasmid

#59355

Purpose

Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-GGA3-GAT

Plasmid

#192259

Purpose

Mammalian expression of GFP-tagged GGA3-GAT

Depositor

Juan Bonifacino

Article

Puertollano et al Cell. 2001 Apr 6;105(1):93-102. doi: 10.

Insert

GGA3-GAT (GGA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
Myc-CD4-mCherry-HA-FRB

Plasmid

#100740

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD4

Use

Tags

FRB, HA, Myc, and mCherry

Expression

Mammalian

Mutation

Engineered to remove large part of extracellular …

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Use

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
FRB-mCherry-Giantin

Plasmid

#186575

Purpose

Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

Giantin (GOLGB1 Human)

Use

Tags

FRB-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-EPSIN 1

Plasmid

#22228

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

please see depositor comment below

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge