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Showing: 161 - 180 of 1088 results

  1. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Use
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  7. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

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  9. pIRESneo2 mycBioID-Tollip

    Plasmid
    #178054
    Purpose
    myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Article
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Use
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178054-plasmid-map-sequence-id-350117

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  11. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Use
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Promoter
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. TIRAP-TAP_IRES_GFP

    Plasmid
    #52740
    Purpose
    Retroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFP
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    3x HA, 3x TEV, and BirA target site
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 161 - 180 of 1088 results