Addgene

Showing: 161 - 180 of 204 results

Results per page:

pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR(T/A) (EIF4EBP1 Human, Synthetic)

Use

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Rap1A FLARE

Plasmid

#134926

Purpose

Rap1A biosensor

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

Rap1A, mCerulean3, RaIGDS RBD, YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Rap1B FLARE

Plasmid

#134927

Purpose

Rap1B biosensor

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

RaIGDS RBD, YPet, Cerulean, Rap1B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
FL tension sensor module

Plasmid

#101170

Purpose

Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

Depositor

Carsten Grashoff

Article

Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

Insert

YPet(short)-FL-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
HP35 tension sensor

Plasmid

#101250

Purpose

Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
stabilized HP35 tension sensor module

Plasmid

#101251

Purpose

Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35st-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
F40 tension sensor

Plasmid

#101252

Purpose

Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-F40-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH666

Plasmid

#169610

Purpose

Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

tetO7-PCMVmin / PPGK / PPGK

Availability

Academic Institutions and Nonprofits only

Enlarge
tagSrc(WT)-mCer3 S208F/V224L

Plasmid

#179138

Purpose

mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA

Use

Tags

mCerulean3 (S208F/V224L)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Lyn Fak biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyso-TORCAR

Plasmid

#64929

Purpose

Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

LAMP1-TORCAR (LAMP1 Human, Synthetic)

Use

Tags

Cerulean, Full-length LAMP1, and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PM-TORCAR

Plasmid

#64930

Purpose

Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

Lyn-TORCAR (EIF4EBP1 Human, Synthetic)

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TORCAR-NLS

Plasmid

#64931

Purpose

Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR-NLS (EIF4EBP1 Human, Synthetic)

Use

Tags

Cerulean, Nuclear localization signal (NLS), and …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-PM-RasAR

Plasmid

#205569

Purpose

Plasma membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

PM-RasAR (RAF1 Human, Synthetic)

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Article

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Insert

FAT FAK biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
VBS mutant of alpha catenin sensor

Plasmid

#71710

Purpose

This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin ΔVBS FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted amino acid residues 316-405 in α -catenin…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
beta catenin binding mutant of alpha catenin sensor

Plasmid

#71711

Purpose

This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin Δβ-cat mutant FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted amino acid residues 48- 163

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge