Showing: 161 - 180 of 204 results
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Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Rap1A FLARE
Plasmid#134926PurposeRap1A biosensorDepositorInsertRap1A, mCerulean3, RaIGDS RBD, YPet
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Rap1B FLARE
Plasmid#134927PurposeRap1B biosensorDepositorInsertRaIGDS RBD, YPet, Cerulean, Rap1B
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FL tension sensor module
Plasmid#101170PurposeExpresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.DepositorInsertYPet(short)-FL-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
HP35 tension sensor
Plasmid#101250PurposeExpresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.DepositorInsertYPet(short)-HP35-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
stabilized HP35 tension sensor module
Plasmid#101251PurposeExpresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.DepositorInsertYPet(short)-HP35st-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
F40 tension sensor
Plasmid#101252PurposeExpresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.DepositorInsertYPet(short)-F40-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pVH666
Plasmid#169610PurposeTier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
UseTagsExpressionMammalianMutationPromotertetO7-PCMVmin / PPGK / PPGKAvailabilityAcademic Institutions and Nonprofits only -
tagSrc(WT)-mCer3 S208F/V224L
Plasmid#179138PurposemCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).DepositorInsertSrc, SsrA
UseTagsmCerulean3 (S208F/V224L)ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorInsertLyn Fak biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
KRas FAK biosensor
Plasmid#78301PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cellsDepositorInsertKRas FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorInsertalpha-catenin FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Human, Synthetic)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-TORCAR
Plasmid#64930PurposePlasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertLyn-TORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PM-RasAR
Plasmid#205569PurposePlasma membrane-targeted RasAR.DepositorInsertPM-RasAR (RAF1 Human, Synthetic)
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorInsertFAT FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 161 - 180 of 204 results