-
Plasmid#92301PurposeExpression of humanized BfCpf1DepositorInserthuBfCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY124 (pcDNA3.1-huBsCpf1)
Plasmid#92300PurposeExpression of humanized BsCpf1DepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ301
Plasmid#205415PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoterDepositorInsertHs1Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBfCas12a
Plasmid#174672PurposeExpresses 6xHis-MBP tagged BfCas12aDepositorArticleInserthuBfCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorArticleInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialMutationPromoterT7Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPxCas12a
Plasmid#174675PurposeExpresses 6xHis-MBP tagged PxCas12aDepositorArticleInserthuPxCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPrCas12a
Plasmid#174674PurposeExpresses 6xHis-MBP tagged PrCas12aDepositorArticleInserthuPrCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL1755
Plasmid#139879PurposeExpression plasmid for naturally occurring 8xMCE protein (without TM region)DepositorInsertDenitrovibrio_acetiphilus_Dacet_0211
UseTagsExpressionBacterialMutationDenitrovibrio_acetiphilus_Dacet_0211(41-988)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_TypeIC_Cascade_cas3_invivo
Plasmid#196400Purposeexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assayDepositorInsertsCas5c
Cas7c
Cas8c
Cas3c
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMO746
Plasmid#117480Purposeupp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanRDepositorInserturacil phosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY006 (pcDNA3.1-hBpCpf1)
Plasmid#69978PurposeExpresses humanized BpCpf1DepositorInserthBpCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePop
Plasmid#50953PurposeExpresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillationsDepositorInsertsE lysis protein
LuxI
LuxR
UseTagsExpressionMutationEarly stop codon introduced in mutated luxR resul…PromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD251
Plasmid#124714PurposeExpression of torS under Ptac and torT under PD59_luxRDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ287 (BsCas12a)
Plasmid#138123PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoterDepositorInsertBsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ287-RVR
Plasmid#138124PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoterDepositorInsertBsCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN512R, K518V and N522RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018289
Plasmid#158822PurposeRare earth element alcohol dehydrogenasesDepositorInsertEnterovibrio_2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only