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Actin-BAF53(a+b)-ires-EGFP

Plasmid

#25461

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53(a+b)

Use

Tags

EGFP

Expression

Mammalian

Mutation

3' of mBAF53a deleted and fused to 3' o…

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pG1-NMGR526

Plasmid

#1122

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)

Use

Tags

GR526

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Δ741-752-GFP

Plasmid

#33119

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of aa 741-752

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
3CB1/cSprouty2

Plasmid

#22096

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (SPRY2 Chicken)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pBi2FC-C-GRF4-P2A:mTagBFP2

Plasmid

#216140

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

GRF4 isoform 1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-MGGAGM-P2A:mCherry

Plasmid

#216142

Purpose

Vector control for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MGGAGM Linker

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-MPK8-P2A:mCherry

Plasmid

#216138

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK8

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-C-MGGAGM-P2A:mTagBFP2

Plasmid

#216141

Purpose

Vector control for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MGGAGM Linker

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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LLP185 pLVP-dCas9-DNMT3a V2

Plasmid

#100936

Purpose

dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

Plasmid

#100941

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)

Use

Tags

1xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

Plasmid

#100944

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)

Use

Tags

3xTy1

Expression

Mammalian

Mutation

F636A, E660A, E725A, R295A

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Use

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFPC1-huNFATc1EE-WT

Plasmid

#24219

Depositor

Jerry Crabtree

Article

Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

Insert

NFATc1 (NFATC1 Human)

Use

Tags

EE and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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