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Plasmid#141101PurposeH2B fused to pr-mEosFP for chromatin visualization and primed conversionDepositorInsertHistone 2B
UseTagspr-mEosFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD_Psra-6::ChR2(H134R)::mCherry
Plasmid#117419PurposeFor selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
UseTagsExpressionWormMutationH134RPromotersra-6Available sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD_Pinx-1::ChR2(H134R)::mCherry
Plasmid#117420PurposeFor selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
UseTagsExpressionWormMutationH134RPromoterinx-1Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (Delta Pest1)
Plasmid#78096Purposemammalian expression of GADD34 Delta Pest1DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationdeletion of PEST1PromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pANY3
Plasmid#112197PurposeThis plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19-NLS-4
Plasmid#54222PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertPCNA-19 NLS (PCNA Human)
UseTagsSV40NLS and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-LacI-LAP
Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19-SV40NLS-4
Plasmid#54223PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40NLS and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP555
Plasmid#222722PurposesfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB511-TP-tagRFP
Plasmid#203482PurposeFor Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertTagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-HO1(51-231)
Plasmid#203486PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.DepositorInsertArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSWD95D293A
Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV.UBI.GFP.miR292T
Plasmid#115972PurposemiR-292 regulated GFP expressionDepositorInsertGFP.miR292T
UseLentiviralTagsGFPExpressionMutationPromoterubiquitinAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIU211
Plasmid#44036DepositorInsertAGA1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJO-7
Plasmid#31992DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT643
Plasmid#8610DepositorInsertbarstar A
UseTagsExpressionBacterialMutationTwo cysteines of barstar changed to alanine, C40A…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
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BFP
Plasmid#201383PurposeControl nucleocytoplasmic transport probeDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pNP568
Plasmid#222725PurposepNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 epsilon
Plasmid#116886PurposeExpresses HA-tagged human 14-3-3 epsilon in mammalian cellsDepositorInsert14-3-3 epsilon (YWHAE Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTagBFP2
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1alpha-CSF2-218-PDGFR
Plasmid#139058PurposeA lentivirus vector to integrate a surface membrane anchored human CSF2 geneDepositorInsertcolony stimulating factor 2 (CSF2) (CSF2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1alpha-IL3-218-PDGFR
Plasmid#139055PurposeA lentivirus vector to integrate a surface membrane anchored human IL3 geneDepositorInsertinterleukin 3(IL3) (IL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNP2270
Plasmid#222733PurposepNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (lacZ)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Diffusive 41kDa
Plasmid#201335PurposeNucleocytoplasmic transport probeDepositorInsertEGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK1221
Plasmid#84220PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
H_NES 41kDa
Plasmid#201346PurposeNucleocytoplasmic transport probeDepositorInsertHIV_NES-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
UseTagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
L_NES 41kDa
Plasmid#201347PurposeNucleocytoplasmic transport probeDepositorInsertAdeno_NES-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha
Plasmid#136434PurposeMammalian expression plasmid for c-myc-tagged PDGFRADepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
Plasmid#132546PurposeExpresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cellsDepositorInsertCIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-GFP-LOVpep
Plasmid#80406PurposeMembrane localized GFP-tagged LOVpep. Expresses in mammalian cellsDepositorInsertStargazin-GFP-LOVpep (CACNG2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP1723
Plasmid#222727PurposesfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds
Plasmid#119059PurposeFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)DepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (TB40/E)
Plasmid#136446PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)
Plasmid#132544PurposeExpresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cellsDepositorInsertCIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GATA1
Plasmid#85693PurposeMammalian expression of mouse GATA1DepositorInsertGATA1 (Gata1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (Merlin)
Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
L_NES 67kDa
Plasmid#201357PurposeNucleocytoplasmic transport probeDepositorInsertAdeno_NES-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only