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Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC01
Plasmid#233643PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagA.DepositorInsertsfGFP with c-terminal VitelloTagA
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMP89b CvTA
Plasmid#141204PurposeChromobacterium violaceum transaminase with TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK005
Plasmid#233642PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagB
UseTags6-HisExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]
Plasmid#211891PurposeBacterial expression of CENP-T[1-242/3D]DepositorInsertCENP-T[1-242/3D] (CENPT Human)
UseTagsExpressionBacterialMutationT11D, T27D, T85DPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01
Plasmid#211890PurposeBacterial expression of CENP-T[1-242/3D]-I3-01DepositorInsertCENP-T[1-242/3D]-I3-01 (CENPT Human, Synthetic)
UseTagsExpressionBacterialMutationT11D, T27D, T85DPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS Citrine
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRC02
Plasmid#114681PurposesfGFP.Strand10-HaloenzymeDepositorInsertsfGFP.Strand10-Haloenzyme
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
UseTagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
UseTagsno tagsExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mTangerine
Plasmid#91763PurposeBacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)DepositorInsertmonomeric Tangerine
UseTagsno tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
UseTagsExpressionBacterialMutationwild typePromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
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CBM9-(PT4)P
Plasmid#171708PurposeCarries CBM9 fusion tagDepositorInsertCellulose Bindind Protein 9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMP89b CvTA V124N
Plasmid#141207PurposeChromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationV124NPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
Plasmid#17859DepositorInsertHaemophilus influenzae heat shock loci U and V
UseTagsExpressionBacterialMutationcoding sequence for residues 108-243 of HslU (the…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mStrawberry
Plasmid#91768PurposeBacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)DepositorInsertmonomeric Strawberry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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CBM9-ID-H1
Plasmid#171709PurposeExpresses SARS-CoV-2 Spike epitopeDepositorInsertSARS-CoV-2 Spike protein (S SARS-CoV-2)
UseTagsCBM-9ExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.a1-554.wt(NarI)
Plasmid#12533DepositorInsertBovine 70 kDa heat shock cognate protein fragment amino acids 1-554
UseTagsExpressionBacterialMutationdeleted carboxy-terminal residues 555-650 of bovi…PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only