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Plasmid#112566PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-GST and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pExp-NHis
Plasmid#112558PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET GFP domain 11 LIC cloning vector (GFP C-11)
Plasmid#30183DepositorTypeEmpty backboneUseTagsBiotin-H6-TEV recognition and GFP domain 11ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tobacco Etch Virus NIa protease (GB0420)
Plasmid#160553PurposepUPD_NIaPro(TEV)DepositorInsertTobacco Etch Virus NIa protease
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
RARS_pNIC-Bio3
Plasmid#153055PurposeExpression construct for producing Avi tagged Arginyl-tRNA synthetaseDepositorInsertRARS (RARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-LIC
Plasmid#62310PurposeE. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEVDepositorTypeEmpty backboneUseTags6xHis, S-tag, and Trx-TagExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp9
Plasmid#167264PurposeBacterial expression of Nsp9DepositorInsertNsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
TNKS2A
Plasmid#42491PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTNKS2A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
NARS_pNIC-Bio3
Plasmid#153054PurposeExpression construct for producing Avi tagged Asparaginyl-tRNA synthetaseDepositorInsertNARS (NARS Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
MPSTA
Plasmid#42482PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMPSTA
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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NUDT14A
Plasmid#42485PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNUDT14A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK1 (3Q5I)
Plasmid#28280DepositorInsertPP-CDPK1 (PB000158.03.0 Plasmodium berghei)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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PG1424PGA
Plasmid#74652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPG1424PGA
UseTagsHis-6-TEVExpressionBacterialMutationPromoterT7Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only