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(P) insUP_ku70 (SBE138) (B)

Plasmid

#187626

Purpose

the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(P) insUP KU70 (B)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_1_WPRE

Plasmid

#209765

Purpose

AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC7

Plasmid

#205060

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 7 aa

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC13

Plasmid

#205063

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 13 aa

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC17

Plasmid

#205061

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 17 aa

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC12

Plasmid

#205068

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 12 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC16

Plasmid

#205069

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 16 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC18

Plasmid

#205070

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 18 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC20

Plasmid

#205071

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 20 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CreiLOV-P2A-FusionRed

Plasmid

#192818

Purpose

Expresses CreiLOV with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT58

Plasmid

#204304

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

3XP3

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT71

Plasmid

#204305

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

ie1

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC9

Plasmid

#205067

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 9 amino acids

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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NeuA.pJL1

Plasmid

#199115

Purpose

In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

Depositor

Michael Jewett

Article

Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

Insert

N-acylneuraminate cytodiylyltransfease (NeuA)

Use

Tags

Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1-myc

Plasmid

#185511

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

deletion of IDILGE in acidic blob

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1

Plasmid

#185510

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

deletion of IDILGE in acidic blob

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB4

Plasmid

#185513

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDILGE replaced with AAAAAA

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB2

Plasmid

#185512

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDIL replaced with AAAAAA

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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