Addgene

Showing: 1761 - 1780 of 8409 results

Results per page:

Sort results by:

pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-FLPX-rc [ChrimsonR-GFP]

Plasmid

#118295

Purpose

AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CAG

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-FLEX-splitTVA-EGFP

Plasmid

#59332

Purpose

Expresses splitTVA-P2A-EGFP

Depositor

Ian Wickersham

Insert

splitTVA950-P2A-EGFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
C453-E02: attB4-CAGp>-attB5

Plasmid

#162972

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

chicken beta-actin/CMV enhancer promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mcherry-WIPI2d-cs-TEV-STREP

Plasmid

#178912

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d

Use

Tags

C term strep and N term mcherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CAG-F14F15S-BFP2-(mCherry)'

Plasmid

#115235

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Venus-pA_U6-BbsI (pE057)

Plasmid

#149351

Purpose

Mammalian expression vector for Venus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir

Plasmid

#178044

Purpose

CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Pten_1523 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-cNanog-pgk-hph

Plasmid

#74907

Purpose

Mammalian expression of cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1

Plasmid

#79912

Purpose

piggyBac transposon vector, Cerulean-2A-sHRPbNLG1

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Cerulean-2A-sHRPbNLG1

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-EGFP/RFP-miR-E1-1int

Plasmid

#19819

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

miRNA

Use

Cre/Lox and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS/Daxx myc S502A/669A

Plasmid

#19283

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

Plasmid

#114112

Purpose

fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Inserts

NanoLuc
Volvox channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-sfGFP-GSAx9-iCre-ERT2

Plasmid

#125748

Purpose

sfGFP and iCreERT2 fused by GSAx9 linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

sfGFP-GSAx9-iCre-ERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO(ChRger1-TS-YFP)

Plasmid

#127245

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO(ChRger3-TS-YFP)

Plasmid

#127242

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge