-
Plasmid#193809PurposeCMV overexpression of ChemoC in mammalian cell linesDepositorInsertChemoC
UseTags10xHisExpressionMammalianMutationmCerulean3:T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoY
Plasmid#193810PurposeCMV overexpression of ChemoY in mammalian cell linesDepositorInsertChemoY
UseTags10xHisExpressionMammalianMutationVenus:A206K, T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-hSyn1-NES-ChemoG-CaM
Plasmid#193817PurposeExpression of ChemoG-CaM in primary neuronsDepositorInsertChemoG-CaM
UseAAVTagsExpressionMutationEGFP:A206K; HT7: L271EPromoterhSyn1Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mRuby3-WPRE
Plasmid#193336PurposeExpresses mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-YPet-WPRE
Plasmid#193335PurposeExpresses YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mAmetrine1.1-WPRE
Plasmid#193333PurposeExpresses mAmetrine1.1 under the TRE promoter in mammalian cellsDepositorInsertmAmetrine1.1
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mNeonGreen-WPRE
Plasmid#193334PurposeExpresses mNeonGreen under the TRE promoter in mammalian cellsDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l BCB
Plasmid#196472PurposeProtein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcherDepositorInsertBCB
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1_F
Plasmid#146314PurposeMammalian Expression of HsAGO1DepositorInsertHsAGO1 (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1963-2505-dsRNAres_AC
Plasmid#148403PurposeInsect Expression of Dmot1_1963-2502-dsRNAresDepositorInsertDmot1_1963-2502-dsRNAres
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot7-D40AE42A_AF
Plasmid#148675PurposeBacterial Expression of HsNot7-D40AE42ADepositorInsertHsNot7-D40AE42A (CNOT7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-HsNot7-NpM-HsNot6_AF
Plasmid#148645PurposeBacterial Expression of HsNot7-HsNot6DepositorInsertHsNot7-HsNot6 (CNOT7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Rtn2b-R60f-mCherry
Plasmid#186607PurposeThird generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherryDepositorInsertHuman Reticulon 2 mutant R60f (RTN2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationc.178insC (R60f)PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Rtn2b-mNeon-TurboID
Plasmid#186612PurposeThird generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboIDDepositorInsertHuman Reticulon 2 isoform B (RTN2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD1-271-mNeon
Plasmid#186601PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD1-135-mNeon
Plasmid#186602PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD1-60-mNeon
Plasmid#186603PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD136-271-mNeon
Plasmid#186604PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mCCK2R-stop
Plasmid#179321PurposeExpress mouse cholecystokinin 2 receptor (untagged) in mammalian cellsDepositorInsertmouse cholecystokinin2 receptor
UseTagsExpressionMammalianMutationstop codon after ORFPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
E42_gControl_dTet_IRES_mTurquoise2
Plasmid#189799PurposeRetroviral delivery of control guide RNADepositorInsertLuciferase gRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
Plasmid#191365PurposeOverexpression of NLS-dPbaCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPbaCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS
Plasmid#191364PurposeOverexpression of NLS-dPba5Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba5Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
Plasmid#191363PurposeOverexpression of NLS-dPba4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba4Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191536PurposeOverexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-dMisCas13b-SNAPf-NLS-3xFlag
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
Plasmid#191366PurposeOverexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm6Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
Plasmid#191362PurposeOverexpression of NLS-dBba2Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dBba2Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
Plasmid#191361PurposeOverexpression of NLS-dPba3Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba3Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS
Plasmid#191359PurposeOverexpression of NLS-dMisCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dMisCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE‐NG‐loop
Plasmid#187057PurposeABEmax-SpCas9-NG-loopDepositorInsertABE‐NG‐loop
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-Electra2
Plasmid#184928PurposeKeratin labeling with blue fluorescent protein Electra2DepositorInsertKeratin-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pElectra1-LaminA
Plasmid#179482PurposeNuclear envelope labeling with blue fluorescent protein Electra1DepositorInsertElectra1-LaminA
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
Plasmid#158771PurposeExpression of synaptotagmin 1 variant with self-cleaving C2 domain.DepositorInsertSynaptotagmin 1 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
L0-AA008
Plasmid#185754PurposeLevel 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).DepositorInsertpAaEF1a (promoter)
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
L0-AA018
Plasmid#185753PurposeLevel 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).DepositorInsertpAaTip1;1 (promoter)
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3a-FruK
Plasmid#186256PurposeOverexpression of FruK for purificationDepositorInsert1-phosphofructokinase (fruK E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
iLID-mRuby3-iTrkA
Plasmid#136510PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)DepositorInsertiLID mRuby3 iTrkA (Ntrk1 Avena sativa, Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
iLID-mRuby3-iTrkB
Plasmid#136511PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)DepositorInsertiLID mRuby3 iTrkB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F
Plasmid#146298PurposeMammalian Expression of HsTNRC6A-delMI-MIIDepositorInsertHsTNRC6A-delMI-MII (TNRC6A Human)
UseTagsExpressionMammalianMutationFive non silent mutations A339T, V417M, R934G, D1…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179075PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Neon(gfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)
Plasmid#178986PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179050PurposeVector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondriaDepositorInsertRPL18Bp-TOM70-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179049PurposeVector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondriaDepositorInsertRPL18Bp-preCOX4-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179048PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisomeDepositorInsertRPL18Bp-PEX25-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179047PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid dropletDepositorInsertRPL18Bp-PDR16-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179046PurposeVector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid dropletDepositorInsertRPL18Bp-ERG6-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)
Plasmid#179045PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosomeDepositorInsertRPL18Bp-Neon(gfp)-Link-ATG8
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179044PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuoleDepositorInsertRPL18Bp-PRC1-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only