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Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BRCA1
Plasmid#115491PurposeEGFP-BRCA1 expression vector. Parton clone BXYDepositorInsertBRCA1 (BRCA1 Human)
UseTagsEGFPExpressionMammalianMutationS1009R (See depositor comments)PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry
Plasmid#161734PurposeDoxycycline-inducible expression of mStrawberryDepositorInsertmStrawberry
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAL4UAS-Luciferase reporter
Plasmid#64125PurposePhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.DepositorInsertGAL4UAS
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGAL4UASAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-rictor
Plasmid#73387PurposeExpresses HA and YFP tagged RictorDepositorInsertRPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
UseTagsHA and YFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorInsertAlpha-Synuclein-His (SNCA Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-EEA1
Plasmid#169067PurposeExpress human EEA1 in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EHD2-mEGFP
Plasmid#45932Purposecaveolar protein, confines caveolae to the plasma membraneDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmonomeric EGFP_FrameN1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
UseTagsExpressionBacterialMutationPromoterPosmY, PsulA, and grpEAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Zeo (637-7)
Plasmid#17449Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, ZeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-U6-GFP-WPRE
Plasmid#200030PurposepiggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-K560-24xGCN4_v4
Plasmid#72229PurposeA fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide arrayDepositorInsertK560 kinesin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-Electra2
Plasmid#184930PurposeTubulin labeling with blue fluorescent protein Electra2DepositorInsertTUBA1B-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2lox-cAMPr
Plasmid#99143PurposeExpresses cAMPr in mammalian cellsDepositorInsertcAMPr
UseCre/LoxTagscpGFPExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP
Plasmid#19444DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCIneo RYR-1
Plasmid#75113PurposeClone encoding rabbit ryanodine receptor in mammalian expression vectorDepositorInsertRyanodine receptor 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only