Addgene

We narrowed to 7,894 results for: sgRNA

Showing: 1741 - 1760 of 7894 results

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pJMP2824

Plasmid

#160671

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pECNUS_P2

Plasmid

#194344

Purpose

sgRNA position entry clone 2

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P1

Plasmid

#194343

Purpose

sgRNA position entry clone 1

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P3

Plasmid

#194345

Purpose

sgRNA position entry clone 3

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P4

Plasmid

#194346

Purpose

sgRNA position entry clone 4

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P5

Plasmid

#194347

Purpose

sgRNA position entry clone 5

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P6

Plasmid

#194348

Purpose

sgRNA position entry clone 6

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P7

Plasmid

#194349

Purpose

sgRNA position entry clone 7

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Use

Tags

Expression

Plant

Mutation

Promoter

Ath U6 promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Use

Tags

NLS-3xHA

Expression

Insect

Mutation

Promoter

act5C

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAL-pgi_NT

Plasmid

#74067

Purpose

pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (NT)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_NT

Plasmid

#74072

Purpose

pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (NT)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUCSh5-2300

Plasmid

#171788

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

rrk1

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAL-pck_NT

Plasmid

#74069

Purpose

pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (NT)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pAL-pgi_T

Plasmid

#74066

Purpose

pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (T)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pJMP2754

Plasmid

#160666

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_T

Plasmid

#74070

Purpose

pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (T)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only