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CAV1-D67G-HA

Plasmid

#27706

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)

Use

Tags

HA

Expression

Mammalian

Mutation

mutant D67G

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pTK157_mCherry-4.1G-CTD

Plasmid

#46359

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHJ386

Plasmid

#20679

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
Δconst-GFP

Plasmid

#33114

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal constant region (amino…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
GFP-ERK2 del174-177

Plasmid

#118297

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 del174-177 (Mapk1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

deletion 174aa-177aa

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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γA3/α1-GFP

Plasmid

#33112

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplaspic domain has been replaced with the cyt…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
VCDstub-GFP

Plasmid

#33122

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
Cenp-B INCENP Δcen mCherry

Plasmid

#45227

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

vsv Cenp-B INCENP Δcen mCherry

Use

Tags

Expression

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pHelper-CRISPRi-sgRNA

Plasmid

#221135

Purpose

Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with dCas9 handle

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119(SpeI)

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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2COX8-BmTyr

Plasmid

#222943

Purpose

mitochondrion-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

2COX8-BmTyr-Flag

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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IgK-BmTyr-KDEL

Plasmid

#222942

Purpose

ER-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

IgK-BmTyr-Flag-KDEL

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-1-SAC1deltaTMD-Fis1tail

Plasmid

#220077

Purpose

SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1313

Plasmid

#217955

Purpose

Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3.1 (H3C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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peMBD2G (pc2399)

Plasmid

#211572

Purpose

Expresses fl. MBD2 GFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMBD2.1R (pc2813)

Plasmid

#211575

Purpose

Expresses MBD2 (aa1-152) RFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MsACR1-pmCerulean3-N1

Plasmid

#204958

Purpose

Expression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

MsACR1

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV (+enhancer)

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-p62 var2

Plasmid

#204575

Purpose

Expresses GFP-p62 var2 in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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