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Plasmid#221954PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE A2
Plasmid#221955PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE C1
Plasmid#221956PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE C2
Plasmid#221957PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE G1
Plasmid#221958PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE T1
Plasmid#221960PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE T2
Plasmid#221961PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CO1 DdCBE C1
Plasmid#221962PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-CO1 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 DdCBE A1
Plasmid#221946PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 DdCBE A2
Plasmid#221947PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2735 (pSpy0C2)
Plasmid#220279Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNAC2):GFP
Plasmid#218574PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 206nt, 207nt from CT to…PromoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNAC1):GFP
Plasmid#218575PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 85nt, 86nt from TA to CGPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNAC3):GFP
Plasmid#218573PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 334nt, 335nt from AG to…PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔMYBs):GFP
Plasmid#218571PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 187nt, 188nt from TG to…PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-100
Plasmid#219408PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(42-10 op)x4-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-422
Plasmid#219410PurposeReporter gene (1 operator) for zinc finger activatorDepositorInsert(42-10 op)x1-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-424
Plasmid#219412PurposeReporter gene (3 operator) for zinc finger activatorDepositorInsert(42-10 op)x3-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only