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pREVtre GFP

Plasmid

#128069

Purpose

Retroviral tet inducible EGFP

Depositor

Oskar Laur

Insert

tet inducible EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

no

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe puro IRES-EGFP

Plasmid

#128037

Purpose

Retroviral vector with IRES-EGFP

Depositor

Oskar Laur

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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prs316 CUP-GFP

Plasmid

#128052

Purpose

Yeast expression vector for N terminal GFP fusions

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLex MCS mCherry Myc

Plasmid

#128051

Purpose

Lentiviral vector with mCherry Myc

Depositor

Oskar Laur

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pE-mApple L5L2

Plasmid

#187690

Purpose

C-Term Gateway cloning tag

Depositor

Mary Beckerle

Insert

mApple

Use

Gateway entry vector

Tags

mApple

Expression

Mutation

WT

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Fidgetin-C-3

Plasmid

#62796

Purpose

Localization: Centrosomes, C1 Cloning Vector, Excitation:N/A, Emission:N/A

Depositor

Michael Davidson

Insert

Fidgetin

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Tomas Santalucia, Anna Planas

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P4-P1R-EGFP

Plasmid

#48348

Purpose

Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Tomas Santalucia, Anna Planas

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Mutation

Contains a Kozak sequence. Does not contain a sto…

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_C2516b

Plasmid

#91085

Purpose

Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Tags

Expression

Mutation

Promoter

At7SL

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP

Plasmid

#13031

Purpose

Mammalian expression vector for cloning your gene fused to EGFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

Enhanced Green Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CFP

Plasmid

#13030

Purpose

Mammalian expression vector for cloning your gene fused to CFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

cyan fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP

Plasmid

#13033

Purpose

Mammalian expression vector for cloning your gene fused to YFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

Yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRFP

Plasmid

#13032

Purpose

Mammalian expression vector for cloning your gene fused to mRFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

Monomer Red Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pHyVec11

Plasmid

#34794

Depositor

Rob Steele

Type

Empty backbone

Use

Hydra expression

Tags

Intergenic region between Hydra replication facto…

Expression

Mutation

Promoter

Hydra vulgaris actin promoter

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-C-terminal YPet

Plasmid

#128027

Purpose

Mammalian expression vector with C terminal YPet tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3- c-terminal 6xMyc

Plasmid

#128023

Purpose

Mammalian expression vector with C terminal 6xMyc tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

6xMyc tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-LIC

Plasmid

#26098

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

Three consecutive Taq promoters, 2 active

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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