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Plasmid#154324PurposesgRNA Destination Vector for SapTrapDepositorInsertSapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS(RA)
Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorInsertBE3-FNLS
UseTags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
UseTags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
UseTagsExpressionWormMutationPromoterPromoter Ultra Pan NeuronalAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3-PGK-Puro
Plasmid#110845PurposeLentiviral vector for dox-inducible expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSRW1JZ
Plasmid#129528PurposeC. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pairDepositorInsertFlincG3
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW2088
Plasmid#154336PurposeCrispr repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::GFP::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R4
Plasmid#61385PurposeExpresses HA tagged TRAIL receptor 4DepositorInsertTRAIL receptor 4 (TNFRSF10D Human)
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R4 leader peptide (aa1-29) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-Puro
Plasmid#110839PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R3
Plasmid#61384PurposeExpresses TRAIL receptor 3DepositorInsertTRAIL receptor 3 (TNFRSF10C Human)
UseTagsHA tag and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R3 leader peptide (aa1-29) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-PGK-Puro
Plasmid#110843PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOD2047-bwmControl
Plasmid#100006PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1947
Plasmid#154339PurposeEnhancer reporterDepositorInsertpes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
UseTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1883
Plasmid#154331PurposesgRNA Target Vector for CRISPRDepositorInsertttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1882
Plasmid#154330PurposesgRNA Target Vector for CRISPRDepositorInsertttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ889
Plasmid#84826PurposeMinimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selectionDepositorInsertsmu-1 (smu-1 Nematode)
UseTagsGFPExpressionBacterial and WormMutationPromoterPsmu-1Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1850
Plasmid#154328PurposesgRNA Target Vector for CRISPRDepositorInsertttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1849
Plasmid#154327PurposesgRNA Target Vector for CRISPRDepositorInsertttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO10-pcDNA3.1-
Plasmid#52508Purposeexpresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO10 (FBXO10 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only