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Plasmid#121685PurposeEF1a-dTEV-protease-mTagBFP2DepositorInsertTEV protease
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_2paCRY2(D387A)
Plasmid#129652Purpose2P-activatable CRY2 with the FAD-disrupting mutationDepositorInsert2paCRY2(D387A)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LEAPTW
Plasmid#205668PurposeshRNAmir backbone for RNA interference, with mTagBFP2 reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LMAPTW
Plasmid#205650PurposeshRNAmir backbone for RNA interference, with mTagBFP2 reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCAPTW
Plasmid#205641PurposeshRNAmir backbone for RNA interference, with mTagBFP2 reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterhCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBAPTW
Plasmid#205659PurposeshRNAmir backbone for RNA interference, with mTagBFP2 reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterCBHAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS109
Plasmid#209330PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR
Plasmid#118470PurposecpBFP-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertblueAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-ATP
Plasmid#193819PurposeCMV overexpression of ChemoB-ATP in mammalian cell linesDepositorInsertChemoB-ATP
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AA082
Plasmid#215997PurposeFragmid fragment: (Selection) fluorescent reporterDepositorHas ServiceCloning Grade DNAInsertStart; mTagBFP2_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-NAD
Plasmid#193824PurposeCMV overexpression of ChemoB-NAD in mammalian cell linesDepositorInsertChemoB-NAD
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6B
Plasmid#176666PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
RNA-Protein Landing Pad
Plasmid#226162PurposeUsed to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expressionDepositorInsertsmTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB
Plasmid#193808PurposeCMV overexpression of ChemoB in mammalian cell linesDepositorInsertChemoB
UseTags10xHisExpressionMammalianMutationEBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoB-CaM
Plasmid#193812PurposeProtein production of ChemoB-CaM in bacteriaDepositorInsertChemoB-CaM
UseTags10xHisExpressionBacterialMutationEBFP2: N39Y, V206KPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2
Plasmid#161559PurposeInducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only