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Showing: 1741 - 1760 of 1977 results

  4. CoV2-Spike-D614G-Alpha

    Plasmid
    #177961
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
    Depositor
    Melanie Ott
    Article
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Insert
    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177961-plasmid-map-sequence-id-348264

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  6. CoV2-Spike-D614G-Gamma

    Plasmid
    #177963
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)
    Depositor
    Melanie Ott
    Article
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Insert
    SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177963-plasmid-map-sequence-id-348258

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  14. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

    Plasmid
    #169076
    Purpose
    Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GGlinker, TEV cleavage site, StrepII and HIS10 Ta…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169076-plasmid-map-sequence-id-416841

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  15. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  16. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  17. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  18. mEos2-PTK2-C-10

    Plasmid
    #57412
    Purpose
    Localization: Focal Adhesion Kinase, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    PTK2 (PTK2 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57412-plasmid-map-sequence-id-213178

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  19. RGS6

    Plasmid
    #38932
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RGS6 (RGS6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pTGD

    Plasmid
    #88894
    Purpose
    Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli
    Depositor
    Keith Tyo
    Article
    Love et al Methods Mol Biol. 2019;1927:37-45. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    88894-plasmid-map-sequence-id-174381

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Showing: 1741 - 1760 of 1977 results