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Plasmid#173230PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173231PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2121
Plasmid#190343PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP–COSMO–PB
Plasmid#169684PurposeCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104WPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-WT
Plasmid#111575PurposeExpresses full length PARP1 in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hISG15
Plasmid#12447DepositorInsertISG15 fragment (ISG15 Human)
UseTagsExpressionMammalianMutationISG15 up to LRLRGGPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Puro
Plasmid#85966PurposeTet/Dox inducible shRNA lentivirus with puromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Xist-PA
Plasmid#26760DepositorInsertXist (Xist Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG-YY1
Plasmid#104396PurposeFLAG-YY1DepositorInsertYY1
UseTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-SCRAMBLE
Plasmid#26701Purpose3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selectionDepositorInsertscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCytochrome C-GFP
Plasmid#41182DepositorInsertCytochrome C
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-Flpo
Plasmid#60663PurposeExpresses pAAV-hSynapsin-FlpoDepositorInsertFlpo
UseAAVTagsExpressionMutationPromoterhSynapsin1Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAF0451 Si74_attP-mCherry donor
Plasmid#193470PurposePromoterless mcherry donor for Si74DepositorInsertSi74_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFPGUSPlus
Plasmid#64401Purposebinary plant vector expressing GFP and GUS, both driven by a 35S promoterDepositorInsertsGFP
GUSPlus
UseBinary vector for plant transformationTagsHisExpressionPlantMutationS65TPromoter35SAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 41kDa
Plasmid#201342PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iAChSnFR
Plasmid#137955PurposeExpresses iAChSnFR under CAG promoterDepositorArticleHas ServiceAAV1 and AAV9InsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-SpCas9-NG
Plasmid#117919PurposeExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.DepositorInsertSpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
UseTagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'3xFlag
Plasmid#208616PurposeEmpty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlagDepositorTypeEmpty backboneUseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with TEV cleavageExpressionBacterialMutationPromoterT7-lacOAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
L4440
Plasmid#1654DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAiTagsT7p, T7p, lacZN, OriF1>>, OriF1<<ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
gag-MCP-pol
Plasmid#211370PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-MCP-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE
Plasmid#198337PurposePB plasmid expressing Halo-tagged PP7 coat proteinDepositorInsertPP7 coat protein 3x Halo-GB1
UsePiggybacTagsHaloTagExpressionMammalianMutationPromoterEf1aAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationV93QPromoterT7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E011 Hs.ARHGAP35
Plasmid#70295PurposeGateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)DepositorInsertARHGAP35 (ARHGAP35 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry2-C1
Plasmid#54563PurposeLocalization: C1 Cloning Vector, Excitation: 587, Emission: 610DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-iCre
Plasmid#89573PurposeExpresses iCre under pCAGDepositorInsertiCre
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3431
Plasmid#144907PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C2 (NR3C2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPR171-Tango
Plasmid#66337PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR171 (GPR171 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FLAG-AKAP-Lbc
Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET17_K560_GFP_His
Plasmid#15219DepositorInsertHuman neuronal kinesin heavy chain (KIF5B Human)
UseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only