166,267 results

1. pUCmini-iCAP-AAV.MaCPNS1

   Plasmid

   #185136

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDS246

   Plasmid

   #43861

   Purpose

   Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

   Depositor

   Keith Joung

   Insert

   mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
   

   Use

   CRISPR

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. TurboID-MCS MSCV-IRES-GFP

   Plasmid

   #207958

   Purpose

   Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

   Depositor

   Timothy Ley

   Article

   Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

   Insert

   TurboID
   

   Use

   Retroviral; Turboid biotin proximity ligase

   Tags

   TurboID

   Expression

   Mammalian

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HPSE-bio-His

   Plasmid

   #53407

   Purpose

   Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   HPSE (HPSE Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG1160 mCherry-FIREtag

   Plasmid

   #205164

   Purpose

   Expresses mCherry -FIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-FIREtag
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM-U6-reRNA-acceptor-2xBbsI

   Plasmid

   #205633

   Purpose

   Cloning and U6-drive expression of ISDra2 TnpB reRNAs

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ultra-Exon1Q145-Myc-A

   Plasmid

   #110487

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-mCherry

   Plasmid

   #207668

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP)

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   MCP-mCherry
   

   Expression

   Mammalian

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcsII-pb5-flag

   Plasmid

   #86769

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 5 (PSMB5 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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