166,289 results

1. ADRB3-DuET

   Plasmid

   #213182

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRB3 (ADRB3 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP339 pGK-dCas9-SunTag

   Plasmid

   #100943

   Purpose

   dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-3XHA-p53

   Plasmid

   #196267

   Purpose

   Mammalian expression of 3xHA tagged wild-type p53

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFPI7

   Plasmid

   #83555

   Purpose

   Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CUP1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Sport6-CD9-pHluorin

   Plasmid

   #130905

   Purpose

   Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD9-pHluorin (CD9 Aequorea victoria, Human)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   phluorin inserted between Asn-50 and Asn-51 in ex…

   Promoter

   CMV

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLJC5-LAMP1-RFP-2xFlag

   Plasmid

   #102931

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   mRFP-2xFlag

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NG-ABE9

   Plasmid

   #240625

   Purpose

   Adenine base editor with narrowed editing window, recognizing NG PAM

   Depositor

   Tingting Jiang

   Article

   Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

   Insert

   TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLC32A1_STOP

    Plasmid

    #161278

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC32A1 (SLC32A1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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