166,289 results

1. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Plasmid

   #179116

   Purpose

   Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site for in-frame fusion
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-DDB1

   Plasmid

   #19918

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-BiFC-Venus

   Plasmid

   #105804

   Purpose

   Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   VN173 (I152L); VC155

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSD080A

   Plasmid

   #218138

   Purpose

   Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

   Plasmid

   #103849

   Purpose

   dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   CMV

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-sgRNA neo

   Plasmid

   #104992

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-MCS1-P2A-MCS2 (Blast)

   Plasmid

   #80921

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AICSDP-34: TNNI1-mEGFP

   Plasmid

   #114411

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPOTv7-blast-mStayGold[E138D]

   Plasmid

   #221049

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mStayGold[E138D]
   

   Use

   Unspecified

   Promoter

   read through

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-EGFP

    Plasmid

    #86639

    Purpose

    Expresses mCherry-eGFP tandem (cytoplasmic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    mCherry-EGFP
    

    Tags

    mCherry-EGFP

    Expression

    Mammalian

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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