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Plasmid#102784PurposePoint mutation in APPL1 that diminishes its interaction with Rab5DepositorInsertAPPL1 (APPL1 Human)
UseTagsmCherryExpressionMammalianMutationchanged asparagine 308 to aspartate; *(see below)PromoterCMVAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry-Raf1
Plasmid#104066PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1DepositorUseTagsExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rab5-L38R-mCherry
Plasmid#102785PurposePoint mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308DDepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationLeucine 38 changed to ArgininePromoterCMVAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1wt-PA-mCherry
Plasmid#138252PurposeWild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).DepositorInsertCBX1-PAmCherry
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx2
Plasmid#116860PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsPAmCherryExpressionMammalianMutationamino acids 169-329 (tandem dimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry TFRC_B
Plasmid#78544PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nanog-t2A-mCherry
Plasmid#127541PurposeExpresses the transactivation domain of mouse Nanog and mCherry via t2A linker.DepositorInsertNanog (Nanog Mouse)
UseTagsExpressionMammalianMutationCodes for the last 110 a.a of NanogPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Paxillin-22
Plasmid#55092PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertPaxillin
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_EMX1-mcherry
Plasmid#159784PurposeS. pyogenes sgRNA collocated with pegRNA targeting human EMX1 geneDepositorInsertspacer of sgRNA targeting EMX1gene (EMX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_HOXD13-mcherry
Plasmid#159793PurposeS. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 geneDepositorInsertspacer of sgRNA targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-CDC42-C-10
Plasmid#55087PurposeLocalization: Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertCDC42 (CDC42 Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRY2(delta2-6)-mCherry
Plasmid#104182PurposeExpresses fusion of CRY2(delta aa 2-6) with mCherryDepositorInsertCRY2(delta2-6)-mCherry
UseTagsExpressionMammalianMutationCRY2PHR with deletion of aa 2-6PromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2(neutral2-6)-mCherry
Plasmid#104181PurposeExpresses fusion of CRY2(neutral aa 2-6) with mCherryDepositorInsertCRY2(neutral2-6)-mCherry
UseTagsExpressionMammalianMutationCRY2PHR K2S M3S D4G K5S K6SPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC-FsCFP-mCherryFP
Plasmid#154836PurposeFrame-shift fluorescent reporter for genomic DNA editingDepositorInserttargeting sequence of VEGF
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Lifeact-7
Plasmid#54846PurposeLocalization: Actin, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsPA-mCherry-miniSOG-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3A2-mCherry
Plasmid#73425PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.DepositorInsertPromoter 3A2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3A2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LSS-SGFP2
Plasmid#112943PurposeProduces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mCherry fusion
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt2(K14A)
Plasmid#86624PurposeExpresses mCherry-tagged human Akt2(K14A) membrane binding mutantDepositorInsertAkt2(K14A) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-VASP-5
Plasmid#55098PurposeLocalization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertVASP
UseTagsmCherry-miniSOGExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Peroxisomes-2
Plasmid#54847PurposeLocalization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsPA-mCherry-miniSOG and Peroximal Targeting Signal…ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only