Showing: 1721 - 1740 of 1875 results
-
Plasmid#129060Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA8 (mouseTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9
Plasmid#129061Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA9 (mouseTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-10
Plasmid#129062Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA10 (mouseTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-11
Plasmid#129063Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA11 (mouseTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12
Plasmid#129064Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA12 (mouseTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV
Plasmid#102422PurposeInducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vectorDepositorInsertTet1 catalytic domain mutant (Tet1 Mouse)
UseTagsHAExpressionMammalianMutationH1620Y and D1622A mutations in Tet1 catalytic dom…PromoterTetO-CMVAvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF9-COMP5AP-AviTag-9xHis
Plasmid#157121PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF9 (TNFRSF9 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ICAM2-COMP5AP-AviTag-9xHis
Plasmid#157174PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertICAM2 (ICAM2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis
Plasmid#157486PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSEMA7A (SEMA7A Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA4D-COMP5AP-AviTag-9xHis
Plasmid#157516PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSEMA4D (SEMA4D Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis
Plasmid#156938PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSEMA4A (SEMA4A Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VCAM1-Fc(DAPA)-AviTag-6xHis
Plasmid#156942PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertVCAM1 (VCAM1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9-KRAB_sgRNA Td2
Plasmid#176264PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_mouse_Adar2_ccC
Plasmid#158117Purposedeletion mADAR2DepositorInsertAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_mouse_Adar2_ccA
Plasmid#158118Purposedeletion mADAR2DepositorInsertAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_mouse_Adar2_ccB
Plasmid#158119Purposedeletion mADAR2DepositorInsertAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A delta Tail
Plasmid#166955PurposeExpresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (1-820aa)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A tail only
Plasmid#166956PurposeExpresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (820-1027aa)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5AR280H
Plasmid#166957PurposeExpresses FLAG-tagged KIF5A R280H protein in pcDNA3.1DepositorInsertKIF5A-R280H (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A-R280HPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP core
Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only
Showing: 1721 - 1740 of 1875 results