Purpose
inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
Insert
dCas9-hudTET1CD, SgRNA cloning site
Use
Tags
HA-Tag, NLS and T2A-mCherry
Expression
Mammalian
Mutation
dCas9 (D10A;H840A), catalytic domain huTET1 inact…
Promoter
CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
Available since
Jan. 25, 2021
Availability
Academic Institutions and Nonprofits only