Skip to main content
Addgene
Showing: 1701 - 1720 of 21049 results

  1. pL0_AC-51183

    Plasmid
    #202108
    Purpose
    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202108-plasmid-map-sequence-id-405432

    Enlarge

  2. pL0_AC-GEF

    Plasmid
    #202107
    Purpose
    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GEF promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI site for…
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202107-plasmid-map-sequence-id-405430

    Enlarge

  3. pL0_AC-47740

    Plasmid
    #202106
    Purpose
    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    47740 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202106-plasmid-map-sequence-id-405429

    Enlarge

  4. pL0_AC-BCA

    Plasmid
    #202105
    Purpose
    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    BCA promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202105-plasmid-map-sequence-id-405428

    Enlarge

  5. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

    Enlarge

  6. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

    Plasmid
    #182381
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters
    Depositor
    Chunghun Lim
    Article
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mCherry
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    HSV TK promoter and SV40 promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182381-plasmid-map-sequence-id-358105

    Enlarge

  7. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid
    #182380
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
    Depositor
    Chunghun Lim
    Article
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    CMV promoter and SV40 promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182380-plasmid-map-sequence-id-358102

    Enlarge

  8. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

    Enlarge

  10. Donor-PIGA

    Plasmid
    #153530
    Purpose
    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    Use
    Donor plasmid
    Tags
    N/A
    Expression
    Mutation
    No mutation
    Promoter
    No promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153530-plasmid-map-sequence-id-319287

    Enlarge

  20. RA35

    Plasmid
    #141126
    Purpose
    Expresses dimeric LacI repressor (LacI-11) under Rhl promoter
    Depositor
    Matthew Bennett
    Article
    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:
    Inserts
    dimeric LacI (LacI-11) (lacI Synthetic)
    AiiA (AQ980_RS12585 )
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    truncated/dimeric version of LacI (missing last 1…
    Promoter
    engineered rhl promoter (responds to C4-HSL) and …
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141126-plasmid-map-sequence-id-292685

    Enlarge

Showing: 1701 - 1720 of 21049 results