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pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
UseTags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-HsSAS6
Plasmid#136839PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Sfi1
Plasmid#136842PurposeMammalian expression of the centrosomal protein hSfi1 N-terminally fused to CLIP-tagDepositorInsertCLIP-hSfi1 (SFI1 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-HsSAS6
Plasmid#136838PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-HsSAS6
Plasmid#136790PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tagDepositorInsertSNAP-SAS6 (SASS6 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b (Glu)
Plasmid#50451Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
UseTagsGstExpressionMammalianMutationchanged Serine 218 to Glutamic Acid, changed Thre…PromoterSV40Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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