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Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BoxB
Plasmid#92004PurposeExpresses firefly luciferase with BoxB sequences in 3' UTRDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_switch
Plasmid#132742PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N02_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9-H840A
Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-26
Plasmid#120005PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-9A
Plasmid#114028PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tagRFP-NLS-HA-Bxb1
Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
UseTagsHA, SV40 NLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N19_switch
Plasmid#132723PurposeT7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N19_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Galphai1-mTurquoise2
Plasmid#69620PurposeThe heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2DepositorInsertGalphai1-mTurquoise2
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM28521
Plasmid#105359PurposeSynthetic biologyDepositorInsertEC1.2en-EC1.1p fusion promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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hSERT-N1-pEYFP
Plasmid#70105Purposemammalian expression of hSERT with C-terminal YFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P09
Plasmid#120020PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenusDepositorInsertmVenus
UsePcr templateTagsmVenusExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]
Plasmid#16283DepositorInsertHB9 promoter (Mnx1 Mouse)
UseMouse TargetingTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only