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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Use

Tags

His and c-myc NLS

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PH-TAPP1

Plasmid

#161985

Purpose

Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

Depositor

Dario Alessi, David Bryant

Article

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Insert

PH-TAPP1 (PLEKHA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAXEF-Rap1-E63(GTP)

Plasmid

#32698

Depositor

Vicki Boussiotis

Article

Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

Insert

RAP1 (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

E63: Consitutively GTP-bound

Promoter

EF promoter

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMV_AA090

Plasmid

#216099

Purpose

Knockout, EF1a-driven Cas12a (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pN1-Lck-GCaMP3

Plasmid

#26974

Depositor

Baljit Khakh

Article

Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

Insert

Lck-GCaMP3

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-PsACR1

Plasmid

#85465

Purpose

Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

Insert

Synthetic construct PsChR1 gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+enhancer)

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGMCtN-TSC10M1-sspB

Plasmid

#49521

Purpose

Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pRDA_722

Plasmid

#216077

Purpose

CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral; Destination

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-MCS-P2A-GFP-PuroR

Plasmid

#107186

Purpose

Base plasmid for cloning gene duplication alleles with eGFP reporter.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ Gal4-GBP2-IRES-GBP7-p65

Plasmid

#50020

Purpose

Multi-purpose expression of a GFP-dependent transcription factor

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD

Use

Multi-purpose expression

Tags

Expression

Mutation

Promoter

CMV-immediate early promoter and SP6 promoter

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP1-10gly-Gal4DBD

Plasmid

#49438

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP1-Gal4 DNA binding domain fusion protein

Use

Tags

Expression

Mammalian

Mutation

C98S (to increase protein solubility), Q3D

Promoter

CAG

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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