Addgene

Showing: 1701 - 1720 of 1977 results

Results per page:

Sort results by:

p21(dPCNA)-mCerulean-NeoR

Plasmid

#215082

Purpose

Expressesp21(dPCNA)-mCerulean in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p21(RxL)-mCerulean-NeoR

Plasmid

#215081

Purpose

Expresses p21(RxL)-mCerulean in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p21(RxL)-mCerulean-PuroR

Plasmid

#215078

Purpose

Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p21(dPCNA)-mCerulean-PuroR

Plasmid

#215079

Purpose

Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_CREBBP_ATG10

Plasmid

#205795

Purpose

Express mEGFP-tagged fusion protein, CREBBP_ATG10 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CREBBP_ATG10 (CREBBP, ATG10 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_ASCC1_MICU1

Plasmid

#205773

Purpose

Express mEGFP-tagged fusion protein, ASCC1_MICU1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASCC1_MICU1 (ASCC1, MICU1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-LINGO2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156900

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LINGO2 (LINGO2 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304_zeo_mmFbxw7(res)

Plasmid

#160106

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.S462Y

Plasmid

#81583

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

H455Q; S462Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.I347M

Plasmid

#81576

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I347M

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.D211N

Plasmid

#82839

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D211N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.R689Q

Plasmid

#81516

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R689Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.A502V

Plasmid

#81494

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A502V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FBXW7_p.P620R

Plasmid

#82780

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P620R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_6

Plasmid

#60255

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GRM4 enhancer (GRM4 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23 C2_10

Plasmid

#60287

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

LINGO2 inactive enhancer (LINGO2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_6

Plasmid

#60292

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GRM4 enhancer (GRM4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C2_10

Plasmid

#60250

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

LINGO2 inactive enhancer (LINGO2 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TRUPATH Triple Gai3

Plasmid

#196050

Purpose

Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphai3-RLuc8 (GNAI3 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p300 core-dCas9-p300 core

Plasmid

#179557

Purpose

encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge