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Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Human, Synthetic)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
SIVu
Plasmid#81089PurposeSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Fascin-C-10
Plasmid#57376PurposeLocalization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFascin (FSCN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmceTagsExpressionMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
Plasmid#106309PurposeThe plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.DepositorInsert6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
UseTags6xflag-3xHaloTag(MtoL)ExpressionMammalianMutationPromoterEF1Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGExpressionMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163031PurposeFLiCRE TF with fast kinetics for neuronal expressionDepositorInsertNrxn3b-MKII-f-hLOV1-TEVcs-tTA
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISH-Gap43
Plasmid#74336PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.DepositorInsertgrowth associated protein 43 (Gap43 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only