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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 flag p53

Plasmid

#10838

Depositor

Thomas Roberts

Article

Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

Insert

p53 (TP53 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (1-220)

Plasmid

#42316

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 (1-220) (VAMP7 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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HA-Akt DN (K179M)

Plasmid

#16243

Depositor

Mien-Chie Hung

Article

Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (K179M)

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-EGFP

Plasmid

#75159

Purpose

Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBad-CA

Plasmid

#105665

Purpose

expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Use

Tags

6x his tag

Expression

Bacterial

Mutation

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ME-Flag

Plasmid

#101860

Purpose

Mds1-Evi-1 flag tagged for transfection into mammalian cells

Depositor

Archibald Perkins

Insert

Mds1-Evi-1 (Mecom Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Expression

Mutation

Promoter

hSyn

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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